New Quantum-Dot-Based Fluorescent Immunosensor for Cancer Biomarker Detection
نویسندگان
چکیده
Cancer antigen 15-3 (CA 15-3) is a biomarker for breast cancer used to monitor response treatments and disease recurrence. The present work demonstrates the preparation application of fluorescent biosensor ultrasensitive detection CA protein tumor marker using mercaptopropionic-acid-functionalized cadmium telluride (CdTe@MPA) quantum dots (QDs) conjugated with antibodies. First, QDs were synthesized by hydrothermal route, resulting in spherical nanoparticles up 3.50 nm diameter. Subsequently, QD conjugates characterized Fourier transform infrared spectroscopy (FTIR), UV absorption, fluorescence. interaction between was studied fluorescence buffer 10-fold diluted commercial human serum. Calibration spiked serum samples gave limit 0.027 U/mL, 1000-fold lower than clinical (25 U/mL 30 U/mL), indicating that this an technique. In addition, rapid obtained within 10 min. selective presence interfering proteins BSA, CEA, CA-125, maximum interference 2% BSA. percent recovery close 100% relative standard deviation (RSD%) values 1.56. Overall, developed provides simple sensitive method monitoring cancer, as well ability detect other molecules interest matrices.
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ژورنال
عنوان ژورنال: Chemosensors
سال: 2022
ISSN: ['2227-9040']
DOI: https://doi.org/10.3390/chemosensors10120518